Pol II caught speeding by single gene imaging

Pol II caught speeding by single gene imaging.

and anterograde transport between the ER and Golgi (Strating & Martens, 2009), are required for Wg secretion by S2 cells. Similarly, knockdown by transgenic RNAi shows that p24 proteins are required for normal levels of Wg secretion in Drosophila wing imaginal discs (Buechling et al, 2011; Port et al, 2011). As with Evi, this requirement seems to be relatively specific, since general secretion ...

Live-cell Imaging of Pol II Promoter Activity to Monitor Gene Expression with RNA reporters

Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters

We describe a ribonucleic acid (RNA) reporter system for live-cell imaging of gene expression to detect changes in polymerase II activity on individual promoters in individual cells. The reporters use strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags) that can be expressed from a promoter of choice. For imaging, the cells are incubated with their ligands...

Intergenic transcription by RNA polymerase II coordinates Pol IV and Pol V in siRNA-directed transcriptional gene silencing in Arabidopsis.

Intergenic transcription by RNA Polymerase II (Pol II) is widespread in plant and animal genomes, but the functions of intergenic transcription or the resulting noncoding transcripts are poorly understood. Here, we show that Arabidopsis Pol II is indispensable for endogenous siRNA-mediated transcriptional gene silencing (TGS) at intergenic low-copy-number loci, despite the presence of two other...

Speeding up SURF Imaging

Any good medical ultrasound imaging system must be able to resolve anatomical structures properly, and simultaneously provide adequate frame rate so that moving structures can be studied with ease. In a conventional imaging system these properties are related by a trade-off, but is it possible to have one and the other by applying more advanced post-processing to acquired RF data? Two new metho...